Modification of the dna sequence between the shine-dalgarno...

Details Modification of the dna sequence between the shine-dalgarno... Modification of the dna sequence between the shine-dalgarno...

C - Chemistry, Metallurgy

12

N

195/1.13, 195/1.

C12N 15/71 (2006.01) C07K 14/55 (2006.01) C12N 1/21 (2006.01) C12N 9/86 (2006.01) C12N 15/11 (2006.01) C12N 15/67 (2006.01)

Patent

CA 1321963

- 1 - HOE 85/F 072 Abstract of the Disclosure: When, in the trp operon of E. coli, the adenosine immedi- ately upstream of the ATG start codon is replaced by cytidine, and guanosine is connected downstream of the start codon ATG, then an Nco I cleavage site which permits cloning is obtained. The operon thus modified results in very strong expression of the gene downstream of the start codon. In addition, it is possible for the formation of .beta.-lactamase to be increased, which results in increased ampicillin resistance, which can be utilized for selec- tion.

507096

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