Modified .alpha.-1,2-fucosyltransferase gene and process for...

Details Modified .alpha.-1,2-fucosyltransferase gene and process for... Modified .alpha.-1,2-fucosyltransferase gene and process for...

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12P 19/18 (2006.01)

Patent

CA 2395076

A gene having been modified in the base sequence of .alpha.-1,2- fucosyltransferase gene originating in Helicobacter pylori; a process for producing .alpha.-1,2-fucosyltransferase by using this gene; and a process for producing a fucose-containing sugar by using a microorganism with the expression of the above enzyme. Thus, a fucose-containing sugar can be economically produced in a large amount by bringing a receptor sugar into contact with a microorganism capable of producing GTP from a GTP precursor and a microorganism capable of producing GDP-fucose from a sugar and GTP in an aqueous medium.

L'invention concerne un gène ayant été modifié dans la séquence de base du gène .alpha.-1,2-fucosyltransférase dérivé des Helicobacter pylori un procédé de production d'.alpha.-1,2-fucosyltransférase au moyen de ce gène, et un procédé de production d'un sucre contenant du fucose au moyen d'un micro-organisme avec l'expression de l'enzyme susmentionnée. Ainsi, un sucre contenant du fucose peut être produit en grande quantité et de façon économique en mettant un sucre récepteur en contact avec un micro-organisme capable de produire de la GTP à partir d'un précurseur de la GTP et un micro-organisme capable de produire de la GDP-fucose à partir d'un sucre et de la GTP dans un milieu aqueux.

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