Modified animal lacking functional pyy gene, monoclonal...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/10 (2006.01) A01K 67/027 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/00 (2006.01) G01N 33/53 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2604423

The present invention provides transgenic animals having a reduced level of expression of peptide YY (PYY) and drug screening platforms using the transgenic animals for identifying agonists and antagonists of PYY. The present invention further provides monoclonal antibodies that bind specifically to full-length PYY[1-36] or the processed form thereof i.e., PYY[3-36] and to diagnostic and drug screening platforms using the monoclonal antibodies. The invention has particular utility for the diagnosis of a predisposition or risk of a subject becoming obese, developing one or more pathologies associated with obesity, or developing a disease/disorder of bone tissue.

L'invention concerne des animaux transgéniques possédant un niveau réduit d'expression du peptide YY (PYY) et des plate-formes de criblage de médicament, utilisant lesdits animaux transgéniques pour l'identification d'agonistes et d'antagonistes de PYY. L'invention porte également sur des anticorps monoclonaux qui se fixent spécifiquement à PYY[1-36] complet ou à la forme traitée de celui-ci, à savoir PYY[3-36] et sur des plate-formes de diagnostic et de criblage de médicament, utilisant lesdits anticorps monoclonaux. L'invention est particulièrement utile pour le diagnostic d'une prédisposition à l'obésité, au développement d'une ou plusieurs pathologies associées à l'obésité, d'une maladie ou d'un trouble du tissu osseux.

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