Modified antibody compositions, methods of making and using...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/18 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) C12N 15/13 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2749339

The present disclosure provides modified antibodies which contain an antibody or antibody fragment (AB) modified with a masking moiety (MM). Such modified antibodies can be further coupled to a cleavable moiety (CM), resulting in activatable antibodies (AAs), wherein the CM is capable of being cleaved, reduced, photolysed, or otherwise modified. AAs can exhibit an activatable conformation such that the AB is more accessible to a target after, for example, removal of the MM by cleavage, reduction, or photolysis of the CM in the presence of an agent capable of cleaving, reducing, or photo lysing the CM. The disclosure further provides methods of making and using such modified antibodies and activatable antibodies.

L'invention concerne des anticorps modifiés qui contiennent un anticorps ou un fragment d'anticorps(AB) modifié par une fraction de masquage (MM). Ces anticorps modifiés peuvent également être couplés à une fraction clivable (CM), ce qui a pour résultat des anticorps activables (AA), la CM pouvant être clivée, réduite, photolysée ou autrement modifiée. AA peut présenter une conformation activable de sorte que AB est plus accessible à une cible après, par exemple, élimination de la MM par clivage, réduction ou photolyse de la CM en présence d'un agent capable de cliver, réduire ou photolyser la CM. L'invention concerne également des procédés de production et d'utilisation des anticorps modifiés et des anticorps activables.

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