C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01H 5/00 (2006.01) C07K 14/325 (2006.01) C12N 5/10 (2006.01) C12N 15/67 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01)
Patent
CA 2080584
A DNA fragment, encoding all or an insecticidally-effective part of a Bacillus thuringiensis crystal protein, is modified by changing A and T sequences to corresponding G and C sequences encoding the same amino acids. These changes are in a plurality of translational codons of the DNA fragment that would otherwise directly or indirectly cause a nuclear event which would negatively control transcription, nuclear accumulation and/or nuclear export of the mRNA. The A and T sequences are changed into G and C sequences in translational codons of at least one region of the gene which, during transcription, would otherwise have thereon a relatively low percentage of RNA polymerase II as compared to another adjacent upstream region of the gene.
Cornelissen Marc
Dockx Jan
Soetaert Piet
Stam Maike
Aventis Cropscience N.v.
Goudreau Gage Dubuc
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