Modified beta-glucosidases with improved stability

Details Modified beta-glucosidases with improved stability Modified beta-glucosidases with improved stability

C - Chemistry, Metallurgy

12

N

C12N 9/42 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 9/24 (2006.01) C12N 15/56 (2006.01) C12P 19/14 (2006.01)

Patent

CA 2735271

Provided are modified beta-glucosidase enzymes, derived from the Trichoderma reesei Cel3A beta-glucosidase, that exhibit improved stability at low pH, low pH and high aeration, low pH and high agitation, or low pH and elevated temperature. Also provided are genetic constructs comprising nucleotide sequences encoding for modified beta-glucosidase enzymes, methods for the production of modified beta- glucosidase enzymes from host strains and the use of the modified beta-glucosidase enzymes in the hydrolysis of cellulose.

La présente invention concerne des enzymes bêta-glucosidases modifiées, dérivées de la bêta-glucosidase de Trichoderma reesei Cel3A, qui présentent une stabilité améliorée à pH bas, pH bas et aération élevée, pH bas et agitation élevée, ou pH bas et température élevée. La présente invention concerne en outre des constructions génétiques comprenant des séquences nucléotidiques codant pour des enzymes bêta-glucosidases modifiées, des procédés pour la production d'enzymes bêta-glucosidases modifiées par des souches hôtes et l'utilisation des enzymes bêta-glucosidases modifiées dans l'hydrolyse de cellulose.

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