Modified beta-lactamase and method for its preparation

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 9/86 (2006.01) A61K 38/46 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2659691

The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E-.DELTA.BL is expressed, and modified by host proteases to E-.DELTA.BL. Appropriate nucleotide molecules, vectors and hosts are also described. E-.DELTA.BL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intestine of patients treated with beta-lactam antibiotics.

La présente invention concerne la modification ciblée après traduction de la métallo-bêta-lactamase par troncature et insertion d'un dipeptide au niveau de l'extrémité du N-terminal afin de réduire l'hétérogénéité du N-terminal dans un système de production d'ADN recombinant. Selon l'invention, une protéine K-T-E-BL est exprimée et elle est modifiée en E-BL par les protéases de l'hôte. L'invention concerne également des molécules nucléotidiques, des vecteurs et des hôtes appropriés. E-BL est utilisable dans une composition pharmaceutique destinée au traitement des effets secondaires défavorables des antibiotiques dans l'intestin de patients traités par des bêta-lactames antibiotiques.

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