Modified carboxypeptidase

Details Modified carboxypeptidase Modified carboxypeptidase

C - Chemistry, Metallurgy

12

N

C12N 9/48 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2281879

A method for transamidating a peptide substrate having a P1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S1', S2 and/or S3 subsite to accommodate a specific peptide substrate.

L'invention porte sur un procédé de transmission d'un substrat peptidique présentant un résidu d'acide aminé P¿1? au moyen d'une chaîne latérale à charge positive. Selon l'invention, le carboxypeptidase Y est modifié pour remplacer au moins un acide aminé présentant une chaîne latérale à charge négative dans un site secondaire S¿1?. En outre, le carboxypeptidase Y modifié peut inclure des résidus d'acide aminé substitué dans un site secondaire S¿1?', S¿2? et/ou S¿3? pour incorporer un substrat peptidique spécifique.

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