Modified cdna for high expression levels of factor viii and...

Details Modified cdna for high expression levels of factor viii and... Modified cdna for high expression levels of factor viii and...

C - Chemistry, Metallurgy

07

K

C07K 14/755 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2541280

The present invention relates to modified DNA sequences coding for biologically active recombinant human factor VIII and its derivatives, recombinant expression vectors containing such DNA sequences, host cells transformed with such recombinant expression vectors, and processes for the manufacture of the recombinant human factor VIll and its derivatives. The invention also covers a transfer vector for use in human gene therapy which comprises such modified DNA sequences. The present invention relates to modified DNA sequences coding for biologically active recombinant human factor VIII and its derivatives, recombinant expression vectors containing such DNA sequences, host cells transformed with such recombinant expression vectors, and processes for the manufacture of the recombinant human factor VIII and its derivatives. The invention also covers a transfer vector for use in human gene therapy which comprises such modified DNA sequences.

L'invention concerne des séquences d'ADN modifié codant pour le facteur VIII humain de recombinaison biologiquement actif et ses dérivés, des vecteurs d'expression de recombinaison contenant lesdites séquences d'ADN, des cellules hôtes transformées au moyen des vecteurs d'expression de recombinaison et des procédés de production du facteur VIll humain de recombinaison et de ses dérivés. L'invention concerne également un vecteur de transfert utilisé dans une thérapie génique humaine comprenant les séquences d'ADN modifiées.

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