Modified chondroitin synthase polypeptide and crystal thereof

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/00 (2006.01) C12N 9/88 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2655186

Disclosed are: (A) a polypeptide comprising an amino acid sequence depicted in SEQ ID NO:2, or (B) a polypeptide which comprises an amino acid sequence having the deletion, substitution or addition of one or several amino acid residues in SEQ ID NO:2 and has a chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce a polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.

La présente invention concerne : (A) un polypeptide qui comprend la séquence d'acides aminés dépeinte sous le SEQ ID NO : 2 ou (B) un polypeptide dont la séquence d'acides aminés présente la délétion, la substitution ou l'addition d'un ou plusieurs résidus acides aminés de SEQ ID NO : 2 et qui a une activité chondroïtine-synthase. L'invention concerne également un acide nucléique qui code pour le polypeptide, ainsi qu'un procédé de production du polypeptide qui comprend au moins les étapes suivantes : (1) expression de l'acide nucléique pour produire un polypeptide ; et (2) collecte du polypeptide produit à l'étape (1). L'invention concerne en outre un cristal du polypeptide qui peut être un cristal monoclinique ou un cristal quadratique.

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