Modified frt recombination sites and methods of use

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2615797

Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

La présente invention se rapporte à des procédés et à des compositions faisant appel à des populations de sites de recombinaison FRT modifiés randomisés pour identifier, isoler et/ou caractériser des sites de recombinaison FRT modifiés. Lesdits sites de recombinaison FRT modifiés recombinogènes peuvent être employés dans une pluralité de procédés permettant de recombiner des polynucléotides d'intérêt, notamment des procédés permettant de recombiner des polynucléotides, d'évaluer l'activité de promoteur, de sélectionner directement des organismes transformés, de réduire au minimum ou d'éliminer l'expression causée par l'intégration aléatoire dans le génome d'un organisme tel qu'un végétal, d'éliminer les polynucléotides d'intérêt, de combiner de multiples cassettes de transfert, de procéder à l'inversion ou à l'excision d'un polynucléotide, et d'identifier et/ou de caractériser des régions de régulation transcriptionnelle.

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