Modified green fluorescent proteins

Details Modified green fluorescent proteins Modified green fluorescent proteins

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C12N 5/00 (2006.01) C12N 15/65 (2006.01) C12P 21/02 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2343586

Modifications in the sequence of Aequorea wild-type GFP provide products having markedly different excitation and emission spectra from corresponding products from wild-type GFP. In one class of modifications, the product derived from the modified GFP exhibits an alteration in the ratio of two main excitation peaks observed with the product derived from wild-type GFP. In another class, the product derived from the modified GFP fluoresces at a shorter wavelength than the corresponding product from wild-type GFP. In yet another class of modifications, the product derived from the modified GFP exhibits only a single excitation peak and enhanced emission relative to the product derived from wild-type GFP.

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