Modified heat shock protein-antigenic peptide complex

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A01N 37/18 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01) A61K 39/002 (2006.01) A61K 39/02 (2006.01) A61K 39/118 (2006.01) A61K 39/12 (2006.01) A61K 39/385 (2006.01) C07K 1/32 (2006.01) C07K 14/47 (2006.01) C12N 5/10 (2006.01) C12N 15/66 (2006.01)

Patent

CA 2321101

The present invention relates to methods for purifying immunogenic, prophylactically and therapeutically effective complexes of modified heat shock proteins noncovalently associated with antigenic peptides of cancer or infected cells. The claimed methods comprise the constructing of a nucleotide sequence encoding a secretable modified heat shock protein, expressing the sequence in an appropriate host cell, recovering the immunogenic complexes from the cell culture and the cells, and purifying the immunogenic complexes by affinity chromatography. Large amounts of such immunogenic complexes can be obtained by large-scale culturing of host cells containing the genetic sequence. The complexes can be used as a vaccine to elicit specific immune responses against cancer or infected cells, and to treat or prevent cancer or infectious diseases.

La présente invention concerne des procédés de purification de complexes de protéines du stress, efficaces sur les plans immunogène, prophylactique et thérapeutique et associés de façon non covalente à des peptides antigènes du cancer ou de cellules infectées. Les procédés de cette invention consistent à élaborer une séquence nucléotidique codant une protéine du stress modifiée sécrétable, exprimant cette séquence dans une cellule hôte appropriée, récupérant les complexes immunogènes de la culture cellulaire et des cellules, et purifiant les complexes immunogènes par affinité chromatographique. Par ailleurs, on peut obtenir de grandes quantités de ces complexes immunogènes par la mise en culture à grande échelle de cellules hôtes contenant la séquence génétique en question. Enfin, on peut utiliser ces complexes comme vaccin pour déclencher des réactions immunitaires spécifiques contre le cancer ou les cellules infectées, et de traiter ou de prévenir le cancer ou les maladies infectieuses.

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