C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/00 (2006.01) C12N 9/16 (2006.01) C12N 9/20 (2006.01) C12N 11/04 (2006.01) C12N 11/14 (2006.01)
Patent
CA 2686917
Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.
L'invention concerne des préparations d'enzymes interfaciales modifiées, en particulier des lipases et des phospholipases, immobilisées sur un support solide, l'enzyme étant entourée d'un micro-environnement hydrophobe, protégé ainsi d'une désactivation et/ou d'une agrégation en présence d'agents hydrophiles, de substrats et/ou de produits de réaction. L'enzyme peut être protégée en étant liée de façon covalente à des groupes lipidiques qui enrobent l'enzyme, ou en étant immobilisée ou incorporée dans un support solide hydrophobe. L'invention concerne également des procédés pour la préparation des enzymes protégées de façon hydrophobe. Les enzymes peuvent être efficacement utilisées dans la préparation d'un biodiesel.
Fasken Martineau Dumoulin Llp
Trans Biodiesel Ltd.
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