Modified lepidopteran receptors and hybrid multifunctional...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A01K 67/027 (2006.01) A61K 48/00 (2006.01) C07K 14/72 (2006.01) C12N 5/10 (2006.01) C12N 15/86 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2296093

In accordance with the present invention, it has been discovered that nuclear receptor proteins isolated from the silk moth bombyx mori (bR) are useful for the regulation of transgene expression. bR is generally thought to be a strong transcriptional regulator within cells of the silk moth. In accordance with the present invention, it has been discovered that bR is also functional in mammalian cells. It has further been discovered that the addition of activation domains to the bR open-reading frame enhances the activity of the ligand modulated regulator to afford high-level transcriptional induction. Further modifications to the bR ligand binding domain result in receptors with unique tranactivational characteristics.

Selon la présente invention, il a été découvert que des protéines réceptrices nucléaires isolées et issues du ver à soie bombyx mori (bR) sont utilisées pour réguler l'expression transgénique. Le bR est généralement censé être un régulateur de transcription efficace à l'intérieur des cellules dudit ver à soie. Selon la présente invention, il a été découvert que le bR est également fonctionnel dans des cellules de mammifères, que l'ajout de domaines activateurs au cadre ouvert de lecture du bR stimule l'activité du régulateur modulé par des ligands et ce, dans le but d'obtenir une induction de transcription de haut niveau. Enfin, l'invention concerne d'autres modifications du domaine de liaison aux ligands du bR qui permettent d'obtenir des récepteurs présentant des propriétés de transactivation uniques.

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