Modified molecular beacons

C - Chemistry – Metallurgy – 07 – H

Patent

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Details

C07H 21/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/533 (2006.01) G01N 33/554 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2530221

Nucleic acid reporters and methods of their use are provided. The nucleic acid reporters include molecular beacons modified with protein transduction domains to facilitate translocation of the nucleic acid reporter across cellular membranes. The nucleic acid reporters are also optionally modified with a targeting signal to direct the nucleic acid reporter to a specific cell, tissue, organ, intracellular region, organelle or vesicle.

L'invention concerne des rapporteurs d'acides nucléiques et des procédés d'utilisation de ceux-ci. Les rapporteurs d'acides nucléiques comprennent des sondes moléculaires beacon modifiées au moyen de domaines de transduction protéiques afin de faciliter la translocation du rapporteur d'acides nucléiques à travers des membranes cellulaires. Les rapporteurs d'acides nucléiques sont également éventuellement modifiés au moyen d'un signal de ciblage permettant de diriger le rapporteur d'acides nucléiques dans une cellule, un tissu, un organe, une région intercellulaire, une organelle ou une vésicule spécifique.

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