C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01) C07J 43/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2117583
2117583 9319078 PCTABS00025 The present invention provides novel modified nucleotide compounds having the general formula: X-n(J)-(d or r)NTP where N is adenosine, guanosine or cytidine; X is H, a fluorophore, a chromophore, a luminescent compound, a ligand or a hapten; n is an integer of 7 or more; and J is a Jeffamine (TM Texaco, Inc.) substituent. These compounds are substantially superior substrates for incorporating label into nucleic acids during enzyme-catalyzed synthesis. Use of a Jeffamine-linked labelled nucleotide derivative as a partial substitute for unlabelled substrate therefore leads to significantly greater incorporation, hence increased label density per unit length of nucleic acid, than the same label joined by a prior art linker. Jeffamine-modified ribo- or deoxyribo-nucleotides are also provided for synthesis of Jeffamine-modified nucleic acids. The invention further provides an improved method of synthesizing labelled nucleic acids. This method provides greater frequency and higher efficiency of label incorporation, thus requiring lower amounts of nucleotide derivative in the reaction mixture.
Flickinger Jeannette L.
Haces Alberto
Mackey Jesse K.
Rashtchian Ayoub
Flickinger Jeannette L.
Haces Alberto
Life Technologies Inc.
Mackey Jesse K.
Ogilvie And Company
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