Modified nucleotides and methods of preparing and using same

Details Modified nucleotides and methods of preparing and using same Modified nucleotides and methods of preparing and using same

C - Chemistry, Metallurgy

07

H

260/229.1, 195/1

C07H 21/00 (2006.01) C07H 19/04 (2006.01) C07H 19/20 (2006.01) C12P 19/34 (2006.01) C12Q 1/18 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1219824

ABSTRACT OF THE DISCLOSURE Compounds having the structure: Image wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C1-position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N9-position of the purine or 7-deazapurine and when B is pyrimidine, it is attached at the Nl-position; wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable com- plex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RN hybrid; wherein the dotted line represents a chemical linkage join- ing B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deaza- purine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; and wherein each of x, y and z represents Image either directly,or when incorporated into oligo- and poly- nucleotides, provide probes which are widely useful. Applications include detection and localization of poly- nucleotide sequences in chromosomes, fixed cells, tissue sections, and cell extracts. Specific applications include chromosomal karyotyping, clinical diagnosis of nucleic acid- containing etiological agents, e.g. bacteria, viruses, or fungi, and diagnosis of genetic disorders.

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