C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2229766
The present invention provides modified oligonucleotides for use in the amplification of a nucleic acid sequence. Amplifications carried out using the modified oligonucleotides result in less non-specific amplification product, in particular, primer dimer, and a concomitant greater yield of the intended amplification product compared to amplifications carried out using unmodified oligonucleotides as primers.
L'invention porte sur des oligonucléotides modifiés, utiles pour l'amplification d'une séquence d'acides nucléiques. Les amplifications effectuées à l'aide des oligonucléotides modifiés donnent un produit d'amplification présentant une non-spécificité moindre, en particulier, un dimère d'amorce, et un rendement correspondant plus grand en produit d'amplification recherché, comparativement à une amplifications effectuée à l'aide d'amorces constituées d'oligonucléotides non modifiés.
Will Stephen Gordon
Young Karen Kwok Ying
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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