C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12N 1/15 (2006.01) C12N 9/26 (2006.01) C12N 15/80 (2006.01)
Patent
CA 2478113
It is intended to provide base sequences participating in the regulation of the expression of a promoter. It is also intended to modify a promoter based on the base sequence data to give a modified promoter having a high expression activity. Namely, a modified promoter constructed by inserting a first DNA fragment containing CCAATNNNNNN (a first base sequence) and a second DNA fragment containing CGGNNNNNNNNNGG (a second base sequence) into a promoter functioning in a fungus, wherein N stands for A (adenine), G (guanine), c (cytosine) or T (thymine).
La présente invention concerne la mise au point de séquences de bases participant à la régulation de l'expression d'un promoteur, et la modification d'un promoteur en se basant sur les données des séquences de bases pour donner un promoteur modifié qui a une activité d'expression importante. Un promoteur modifié est produit par insertion d'un premier fragment d'ADN contenant CCAATNNNNNN (première séquence de bases) et un second fragment d'ADN contenant CGGNNNNNNNNNGG (seconde séquence de bases) dans un promoteur qui a une action sur un champignon, N représentant A (adénine), G (guanine), C (cytosine) ou T (thymine).
Suzuki Kanako
Tsukagoshi Norihiro
Amano Enzyme Inc.
Nelligan O'brien Payne Llp
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