Modified proteins and methods of their production

Details Modified proteins and methods of their production Modified proteins and methods of their production

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) C07K 1/00 (2006.01) C07K 1/22 (2006.01) C12N 9/00 (2006.01) C12N 9/96 (2006.01) C12P 21/02 (2006.01) C12P 21/06 (2006.01) C12N 15/55 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2236948

The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity.

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