Modified proteins and process for the production thereof

Details Modified proteins and process for the production thereof Modified proteins and process for the production thereof

C - Chemistry, Metallurgy

12

N

195/123, 530/3.0

C12N 9/00 (2006.01) C12N 9/16 (2006.01)

Patent

CA 1201990

ABSTRACT Disclosed is a modified enzyme like protein and a process for the production thereof. In one embodiment, a native protein is grossly denatured. Subsequently, the grossly denatured protein is partially renatured to form a partially denatured protein. The partially denatured protein is contacted with an inhibitor of a model enzyme whose biological catalytic activity is to be modeled. The partially denatured native protein in the protein-inhibitor complex is cross-linked to produce a new enzyme like modified protein. The enzyme like modified protein shows the enzymatic catalytic behavior characteristics of the model enzyme. - 43 -

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