Modified protozoan expressing at least two variable surface...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/113 (2010.01) A61K 39/002 (2006.01) C07K 1/22 (2006.01) C07K 14/44 (2006.01) C12N 1/11 (2006.01) C12N 15/30 (2006.01)

Patent

CA 2745470

Modified protozoa parasites comprising simultaneous expression on its surface of at least two variable surface proteins (VSP). The modified protozoa may also simultaneously express the complete repertoire of variable surface proteins. Protozoa show reduced expression of Dicer, RNA-dependant RNA-polymerase (RdRP) enzymes or both, wherein the RdRP gene and/or the Dicer gene has been silenced. The protozoan may be any protozoan showing an antigenic variation mechanism.

L'invention concerne des protozoaires parasites modifiés capables d'expression simultanée sur leur surface d'au moins deux protéines de surface variables (VSP). Les protozoaires peuvent également simultanément exprimer le répertoire complet des protéines de surface variables. Les protozoaires présentent une expression réduite d'enzymes Dicer, ARN-polymérase dépendante de l'ARN (RdRP) ou des deux, le gène de RdRP et/ou le gène de Dicer ayant été rendus silencieux. Les protozoaires peuvent être des protozoaires quelconques présentant un mécanisme de variation antigénique.

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