Modified random primers for probe labeling

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2443894

Methods are provided for labeling nucleic acid molecules for use in hybridization reactions, and kits employing these methods. The level of labeling is increased by including one or more reactive modifications, such as amine-modifications, into the primers used to initiate synthesis of the nucleic acid molecule. For instance through random-primed reverse transcription. Also provided are modified random primers (such as amine- modified random primers) useful in these methods, labeling and hybridization kits comprising such primers, labeled nucleic acid molecules and mixtures of molecules, and methods for using them.

L'invention concerne des procédés de marquage de molécules d'acide nucléique destinées à être utilisées dans des réactions d'hybridation, ainsi que des kits utilisant ces procédés. Le niveau de marquage est accru en incluant une ou plusieurs modifications de réaction, telles que des modifications de l'amine, dans les amorces utilisées pour amorcer la synthèse de la molécule d'acide nucléique, par exemple, par transcription inverse amorcée de manière aléatoire. L'invention concerne en outre des amorces aléatoires modifiées (telles que des amorces aléatoires modifiées par une amine) utilisées dans ces procédés, des kits de marquage et d'hybridation comprenant de telles amorces, des molécules d'acide nucléique marquées et des mélanges de telles molécules, ainsi que des procédés utilisant celles-ci.

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