Modified serum albumin with reduced affinity for nickel and...

A - Human Necessities – 01 – N

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Details

A01N 43/04 (2006.01) A61K 8/34 (2006.01) A61K 8/64 (2006.01) A61K 47/42 (2006.01) A61Q 5/02 (2006.01) A61Q 5/12 (2006.01) A61Q 19/00 (2006.01) A61Q 19/10 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C07K 14/765 (2006.01)

Patent

CA 2416097

A modified serum albumin is provided which has been modified in the n-terminal region or binding region (VI), such as through a truncation of at least one amino acid at the n-terminal end, so that it exhibits reduced or eliminated binding of trace metals such as nickel and/or copper. Other suitable modifications to this binding region include mutations such as an elongation or insertion which will be sufficient to disrupt the trace metal binding which is highest at this site. The modified albumin of the present invention is advantageous in that it is binding to trace metals is reduced or eliminated, and it can thus be used more safely and effectively than unmodified albumin with a reduced or eliminated likelihood of causing an allergic reaction to the trace metal in the human being treated with the albumin composition.

La présente invention concerne une albumine sérique modifiée dont la région n-terminale ou le domaine de liaison (VI) a été modifié, comme, par exemple, par la troncature d'au moins un acide aminé à l'extrémité n-terminale, de façon qu'elle présente une liaison nulle ou réduite aux métaux-traces comme le nickel et/ou le cuivre. D'autres modifications adéquates apportées à ce domaine de liaison incluent des mutations telles qu'une élongation ou une insertion suffisantes pour rompre la liaison au métal-trace la plus élevée sur le site. L'albumine modifiée de l'invention est avantageuse en ce que sa liaison aux métaux-traces est réduite ou éliminée et qu'elle peut par conséquent être utilisée de façon plus sûre et plus efficace que l'albumine non modifiée, avec un risque nul ou réduit de provoquer une réaction allergique au métal-trace chez l'être humain traité à l'aide de ladite composition d'albumine.

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