Modified steptococcus pneumonia pneumolysin (ply) polypeptides

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/315 (2006.01) A61K 39/09 (2006.01) A61P 31/04 (2006.01) C07K 16/12 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2748149

This disclosure re-lates to modified Streptococcus pneumonia pneumolysm (PLY) pro-teins which lack hemolytic activity and can be used as immunogens in an immunogenic composition or vac-cine against invasive pneumococcol diseases caused by S. pneumonia The modified pneumolysm proteins com-prise ammo acid substitutions at thre-onine 65, glycine 293 and cysteine 428 Nucleic acids, polypeptides en-coded thereby, compositions contain-ing the same, methods for using such nucleic acids, polypeptides and com-positions are also provided

L'invention concerne les protéines modifiées de pneumolysine de streptococcus pneumoniae manquant d'activité hémolytique et pouvant être utilisées comme immunogènes dans une composition immunogène ou un vaccin contre les maladies pneumococciques invasives causées par la S. pneumoniae. L'invention concerne également les protéines modifiées de pneumolysine comprennent des substitutions d'acides aminés au niveau des acides nucléiques de thréonine 65, de glycine 293 et de cystéine 428, les polypeptides ainsi codés, les compositions contenant ceux-ci, les procédés d'utilisation de ces acides nucléiques, les polypeptides et les compositions.

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