Modified tobacco 5-epi-aristolochene synthases

Details Modified tobacco 5-epi-aristolochene synthases Modified tobacco 5-epi-aristolochene synthases

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/00 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2342053

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e.g., the three-dimensional coordinates of tobacco 5-epi- aristolochene synthase, with or without a substrate bound in the active site.

L'invention concerne des nouvelles synthases ainsi que les acides nucléiques correspondants, codant ces synthases. De telles synthases possèdent une poche de site actif comprenant des restes d'acides aminés clés, modifiés pour produire des intermédiaires et produits recherchés de réaction terpénique. On a conçu des modifications de synthases en fonction, par exemple, des coordonnées tridimensionnelles de la 5-épi-aristolochène synthase du tabac, avec ou sans substrat lié au site actif.

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