Modified variant bowman birk protease inhibitors

Details Modified variant bowman birk protease inhibitors Modified variant bowman birk protease inhibitors

C - Chemistry, Metallurgy

07

K

C07K 14/81 (2006.01) C07K 7/06 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2740197

The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care.

La présente invention concerne des protéines variantes modifiées dinhibiteur de la protéase Bowman-Birk (BBPI), qui comprennent des peptides qui se lient aux protéines cibles, et qui sont encore modifiées pour présenter une plus grande activité dinhibiteur de protéase et/ou pour être produites en de plus grands rendements que les BBPI non modifiés. Linvention comprend des constructions de polynucléotide et des vecteurs dexpression contenant des séquences de polynucléotide, qui codent les BBPI variants modifiés, les cellules hôtes transformées, qui expriment et produisent les BBPI variants modifiés, les protéines variantes modifiées BBPI et les procédés de préparation et dutilisation des BBPI variants modifiés dans le secteur des soins personnels.

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