Modified yeast cells expressing exogenous xylose isomerase

C - Chemistry – Metallurgy – 07 – H

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C07H 21/04 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 9/90 (2006.01)

Patent

CA 2524387

Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications', include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.

Selon l'invention, des cellules de levure sont transformées à l'aide d'un gène de xylose isomérase exogène. Des modifications génétiques supplémentaires améliorent la capacité des cellules transformées à fermenter le xylose en éthanol ou autres produits de fermentation désirés. Parmi lesdites modifications, on trouve la délétion d'au moins un gène d'aldose réductase spécifique ou non spécifique, la délétion d'au moins un gène de xylitol déshydrogénase et/ou la surexpression de la xylulokinase.

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