Modifying cell metabolism via extra-cellular nucleases

Details Modifying cell metabolism via extra-cellular nucleases Modifying cell metabolism via extra-cellular nucleases

C - Chemistry, Metallurgy

12

N

95/45, 195/34.4

C12N 5/04 (2006.01) C12N 15/01 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 1312298

MODIFYING CELL METABOLISM VIA EXTRA-CELLULAR NUCLEASES ABSTRACT OF THE INVENTION This invention involves a method of altering and regulating the gene expression of cells, by means of contacting the cells with a nuclease capable of degrading extra-cellular DNA and/or RNA. The nuclease will degrade the extra-cellular nucleic acids (NA) into nucleotides or oligonucleotides which are too short to have substantial affinity for the chromosomal DNA. By means of this method, cultures of cells have been created with desirable properties, including greater phenotypic uniformity and higher levels of cell reproduction. This invention also comprises a culture of cells which has been treated by this method, and cells descended from cells which have been treated according to this method.

562079

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