Modifying insect cell gylcosylation pathways with...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/86 (2006.01) A01K 67/033 (2006.01) A01N 63/00 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C12N 7/01 (2006.01) C12N 9/10 (2006.01) C12N 15/866 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2264953

Disclosed are a variety of recombinant baculovirus vectors, and host insect cells, which comprise at least one oligosaccharide processing enzyme gene. The vectors and cells may optionally comprise other heterologous structural genes, including further protein processing enzymes. Methods of making and using the recombinant baculoviruses and vectors are provided, including their uses in recombiant protein production and as insecticides.

La présente invention concerne divers vecteurs d'expression recombinés et des cellules hôtes d'insectes, compreannt au moins un gène enzymatique de maturation de l'oligosaccharide. Les vecteurs et les cellules peuvent facultativement comprendre d'autres gènes de structure hétérologues, y compris des enzymes de maturation de protéines supplémentaires. On décrit les procédés de fabrication et d'utilisation des baculovirus et des vecteurs recombinés, et l'on décrit notamment leurs utilisations dans la production de protéines recombinées et comme insecticides.

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