Modular dna-binding domains and methods of use

Details Modular dna-binding domains and methods of use Modular dna-binding domains and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/113 (2010.01) C07K 14/00 (2006.01) C07K 14/195 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2749305

The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.

La présente invention porte sur des procédés de reconnaissance sélective d'une paire de bases dans une séquence d'ADN par un polypeptide, sur des polypeptides modifiés qui reconnaissent spécifiquement une ou plusieurs paires de bases dans une séquence d'ADN et sur un ADN qui est modifié de telle sorte qu'il peut être spécifiquement reconnu par un polypeptide et sur des utilisations du polypeptide et de l'ADN dans un ciblage d'ADN spécifique ainsi que sur des procédés de modulation de l'expression de gènes cibles dans une cellule.

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