Modular protein libraries and methods of preparation

Details Modular protein libraries and methods of preparation Modular protein libraries and methods of preparation

C - Chemistry, Metallurgy

07

K

C07K 1/04 (2006.01) A61K 38/04 (2006.01) C07K 14/52 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2301846

Novel proteins and libraries comprising them are disclosed. The proteins comprise one or more functional protein modules from different parent protein molecules. The proteins and libraries are exemplified by the preparation of cross-over chemokines comprising various combinations of peptide segments derived from RANTES, SDF-1 and vMIP-I and vMIP-II. The proteins and libraries are extremely pure and can be provided in non-limiting high yields suitable for diagnostic and high-throughput screening assays.

L'invention concerne de nouvelles protéines et les banques qui les contiennent. Ces protéines renferment un ou plusieurs modules protéiniques fonctionnels provenant de différentes molécules protéiniques parents. Ces protéines et ces banques sont illustrées par la préparation de chémokines à enjambement comprenant diverses combinaisons de segments peptidiques dérivés de RANTES, SDF-1, et MIP-I et vMIP-II. Ces protéines et ces banques sont en outre extrêmement pures et peuvent convenir, de par leur forte capacité, à des méthodes diagnostiques et des analyses à rendement élevé.

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