Modulation of gene expression through endogenous small rna...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01)

Patent

CA 2726052

Gene expression can be selectively regulated by endogenous miRNAs that target promoters of genes. Altering of the activity of these promoter-targeting miRNAs with single- stranded complementary oligonucleotides that bind the miRNA causes modulation of expression of the target gene. Endogenous miRNAs that modulate expression of target genes can be identified by (a) evaluating an endogenous miRNA for complementarity to a target gene promoter; and (b) determining that the complementary miRNA modulates expression of the target gene.

L'expression génique peut être sélectivement régulée par des ARNmi endogènes qui ciblent des promoteurs de gènes. L'altération de l'activité de ces ARNmi ciblant un promoteur avec des oligonucléotides complémentaires simple brin qui se lient à l'ARNmi provoque une modulation de l'expression du gène cible. Les ARNmi endogènes qui modulent l'expression de gènes cibles peuvent être identifiés par (a) évaluation d'un ARNmi endogène pour complémentarité à un promoteur de gènes cible ; et (b) détermination du fait que l'ARNmi complémentaire module l'expression du gène cible.

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