Modulation of molecular interaction sites on rna and other...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G06F 19/18 (2011.01) C12N 15/11 (2006.01)

Patent

CA 2331726

Methods for the identification of compounds which modulate, either inhibit or stimulate, biomolecules are provided. Nucleic acids, especially RNAs are preferred substrates for such modulation. The present methods are particularly powerful in that they provide novel combinations of techniques which give rise to compounds, usually "small" organic compounds, which are highly potent modulators of RNA and other biomolecular activity. In accordance with preferred aspects of the invention, very large numbers of compounds may be tested essentially simultaneously to determine whether they are likely to interact with a molecular interaction site and modulate the activity of the biomolecule. Pharmaceuticals, veterinary drugs, agricultural chemicals, industrial chemicals, research chemicals and many other beneficial compounds may be identified in accordance with embodiments of this invention.

L'invention concerne des procédés servant à identifier des composés qui modulent, inhibent ou stimulent des biomolécules. Des acides nucléiques, en particulier l'ARN, sont les substrats préférés pour cette modulation. Ces procédés sont particulièrement puissants, en ce qu'ils fournissent de nouvelles combinaisons de techniques qui produisent des composés, habituellement de petits composés organiques, qui sont des modulateurs très puissants d'ARN et d'une autre activité biomoléculaire. Conformément aux aspects préférés de l'invention, on peut tester essentiellement simultanément de très grands nombres de composés, de façon à déterminer s'ils sont susceptibles d'interagir avec un site d'interaction moléculaire, et de moduler l'activité de la biomolécule. On peut identifier des produits pharmaceutiques et vétérinaires, des produits chimiques agricoles, industriels, et de recherche, et beaucoup d'autres composés bénéfiques selon les réalisations de cette invention.

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