C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.18, 195/1.
C12N 15/75 (2006.01) C12N 1/21 (2006.01) C12N 9/54 (2006.01) C12N 15/57 (2006.01) C12N 15/87 (2006.01) C12N 15/90 (2006.01)
Patent
CA 1339100
Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions.
560126
Mulleners Leonardus Johannes Sofie Marie
Van Der Laan Johannes Cornelis
Van Eekelen Christiaan Albertus Gerardus
Genencor International Inc.
Gist - Brocades N.v.
Osler Hoskin & Harcourt Llp
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