Molecular cloning of genomic and cdna sequences encoding...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A01K 67/02 (2006.01) A01K 67/027 (2006.01) A61K 38/17 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07K 14/705 (2006.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2048993

2048993 9010699 PCTABS00002 The subject invention provides an isolated nucleic acid molecule which comprises nucleic acid encoding a polypeptide which has the biological activity of a receptor for picornavirus and in addition, provides a purified polypeptide which has this activity. The subject invention also provides a method for inducing the production of a polypeptide which includes the use of expression vectors in a host vector system. Therapeutic compositions comprising purified polypeptides which have the biological activity of a receptor for picornavirus are also provided for as are methods of treating and preventing human poliovirus infection. Further, the subject invention provides a method of producing a transgenic animal expressing human picornavirus. The subject invention provides a method of testing the efficacy of a picornavirus vaccine which comprises administering the vaccine to a transgenic animal described above and determining whether the resulting transgenic animal is protected from infection by human picornavirus. Lastly, a method of testing the virulence of a picornavirus vaccine is provided.

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