Molecular cloning of the genes responsible for collagenase...

Details Molecular cloning of the genes responsible for collagenase... Molecular cloning of the genes responsible for collagenase...

C - Chemistry, Metallurgy

12

N

C12N 15/57 (2006.01) A61K 38/48 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 9/52 (2006.01) C12N 15/63 (2006.01) C12S 3/24 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2138948

2138948 9400580 PCTABS00030 Genetically-engineered E. coli carrying vectors containing an insert that codes for non-native and native forms of Clostridium histolyticum collagenase. The recombinant DNA segments coding for collagenase are expressed efficiently in the transformed cells to yield enzymatically active and immunologically cross-reactive collagenase. Genetically-engineered collagenase is used for isolation of pancreatic islets, for isolation of endothelial cells, for the isolation of dispersed tumor cells, or for the treatment of "slipped disc".

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