Molecular conjugate

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/00 (2006.01) C07C 323/60 (2006.01) C07K 16/42 (2006.01) C07K 16/44 (2006.01) G01N 33/532 (2006.01)

Patent

CA 2631005

A method is disclosed for making a conjugate of two molecules using a hydrazide thiol linker. In a particular working embodiment, an Fc-specifÊc antibody- enzyme conjugate is made using the method and demonstrated to provide exceptional staining sensitivity and specificity in immunohistochemical and in situ hybridization assays.

La présente invention concerne un procédé pour préparer un conjugué de deux molécules en utilisant un groupe de liaison hydrazide-thiol. Dans un mode de réalisation pratique particulier, un conjugué anticorps spécifique Fc-enzyme est préparé en utilisant le procédé et il présente une sensibilité de coloration et une spécificité exceptionnelles en immunohistochimie et dans des dosages d'hybridation in situ.

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