Molecular detection apparatus and method using optical...

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G01N 21/76 (2006.01) C12Q 1/68 (2006.01) G01N 21/64 (2006.01) G01N 21/77 (2006.01) G01N 33/543 (2006.01) G02B 6/10 (2006.01)

Patent

CA 2251942

A target molecule at a binding site is detected using an optical waveguide (10) having a surface (12) proximate to the binding site (14), and a waveguide detector (36) coupled to the optical waveguide (10). An incident light beam is applied to the binding site (14) along an axis transverse to the surface of the optical waveguide. The incident light beam impinges an optical indicator associated with the target molecule (20) to form secondary light (34) which is coupled into the optical waveguide. The secondary light (34) is detected by the waveguide detector to thereby detect the target molecule.

La présente invention consiste à détecter une molécule cible se trouvant en un site de liaison en utilisant un guide d'ondes optiques (10) dont une surface (12) est située au voisinage du site de liaison, un détecteur de guide d'ondes (36) étant couplé au guide d'ondes optiques (10). Le site de liaison (14) est soumis à un faisceau lumineux incident selon un axe transversal par rapport à la surface du guide d'ondes optiques. Rencontrant un indicateur optique associé à la molécule cible (20), le faisceau lumineux incident génère une lumière secondaire (34) qui se couple dans le guide d'ondes optiques. La détection de la lumière secondaire (34) par le détecteur de guide d'ondes permet alors de détecter la molécule cible.

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