Molecular diagnostics amplification system and methods

Details Molecular diagnostics amplification system and methods Molecular diagnostics amplification system and methods

C - Chemistry, Metallurgy

12

M

C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2634735

The present invention relates to automated devices and methods for the amplification of segments of nucleic acid in a convenient and portable manner. A single-use nucleic acid amplification device for producing an amplicon includes a housing and an amplification chamber. The chamber includes an ingress with a first reversible seal, an egress with a second reversible seal, a sealable sample entry orifice, and a first wall forming a portion of the chamber. The first wall includes a thermally conductive material and includes an interior surface and an exterior surface. The exterior surface includes a heating circuit and a temperature sensor. The sample entry orifice permits a sample of nucleic acid to enter the amplification chamber. The ingress is connected to a first conduit along with a pneumatic pump and a fluid pouch. The egress is connected to a second conduit permitting egress of the amplicon from the amplification chamber.

La présente invention a trait à des dispositifs automatiques et des procédés d~amplification de segments d~acide nucléique pratiques et portables. Un dispositif d~amplification d~acide nucléique à usage unique pour la production d~un amplicon comprend un boîtier et une chambre d~amplification. La chambre comporte une entrée avec un premier scellement réversible, une sortie avec un second scellement réversible, un orifice d~entrée d~échantillon scellable et une première paroi qui forme une partie de la chambre. La première paroi présente un matériau thermoconducteur, une surface intérieure et une surface extérieure. La surface extérieure comporte un circuit chauffant et un capteur de température. L~orifice d~entrée d~échantillon permet à un échantillon d~acide nucléique de pénétrer dans la chambre d~amplification. L~entrée est reliée à un premier conduit, ainsi qu~à une pompe pneumatique et une poche de fluide. La sortie est reliée à un second conduit permettant à l~amplicon de quitter la chambre d~amplification.

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