Molecular identification of aspergillus species

Details Molecular identification of aspergillus species Molecular identification of aspergillus species

C - Chemistry, Metallurgy

12

N

C12N 15/31 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2484744

Novel techniques for the detection of Aspergillus in samples are disclosed. These techniques relate to PCR amplification and/or detection of Aspergillus ITS1 rDNA sequences, and the identification of particular species of Aspergillus by detecting differences in the ITS1-V1, ITS-V2, ITS-V3, ITS-V4, and ITS-V5 nucleic acid sequences of Aspergillus. The highly variable regions of the ITS1 rDNA sequences are particularly useful in distinguishing, for example, Aspergillus clavatus, Aspergillus granulosus, Aspergillus sydowii, Aspergillus flavipes, Aspergillus restrictus, Aspergillus versicolor, Aspergillus wentii, and Aspergillus chevalieri. In particular embodiments, the sequence differences are also able to distinguish among variants of particular species, such as Aspergillus granulosus CBS 119.5A, Aspergillus granulosus strain NRRL 1932, Aspergillus sydowii strain NRRL 250, Aspergillus sydowii strain NRRL 4768, Aspergillus sydowii strain CUHI, Aspergillus sydowii strain CUH2, Aspergillus sydowii strain CUH7, and Aspergillus sydowii strain CUH8.

Cette invention concerne des nouvelles techniques de détection d'Aspergillus dans des échantillons. Ces techniques concerne l'amplification de la réaction enchaîne de la polymérase et/ou la détection de séquences ITS1 d'ADNr d'Aspergillus, ainsi que l'identification d'espèces particulières d'Aspergillus par détection de différences dans les séquences d'acides nucléiques ITS1-V1, ITS-V2, ITS-V3, ITS-V4, et ITS-V5 d'Aspergillus. Les régions à forte variabilité des séquences ITS1 d'ADNr conviennent tout particulièrement bien pour distinguer, par exemple, entre Aspergillus clavatus, Aspergillus granulosus, Aspergillus sydowii, Aspergillus flavipes, Aspergillus restrictus, Aspergillus versicolor, Aspergillus wentii, et Aspergillus chevalieri. Dans des modes de réalisation particuliers, les différences entre séquences permettent également de distinguer entre des variantes d'espèces particulières telles que Aspergillus granulosus CBS 119.5A, Aspergillus granulosus souche NRRL 1932, Aspergillus sydowii souche NRRL 250, Aspergillus sydowii souche NRRL 4768, Aspergillus sydowii souche CUHI, Aspergillus sydowii souche CUH2, Aspergillus sydowii souche CUH7, and Aspergillus sydowii souche CUH8.

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