Molecular in vitro diagnosis of breast cancer

Details Molecular in vitro diagnosis of breast cancer Molecular in vitro diagnosis of breast cancer

C - Chemistry, Metallurgy

40

B

C40B 40/06 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01)

Patent

CA 2711396

The invention relates to the use of a multiplicity of polynucleotide probe sets, said multiplicity of polynucleotide probe sets consisting in a combination of pools of polynucleotide probe sets, each polynucleotide probe set containing at least one polynucleotide probe chosen among a library of nucleic acid sequences, the polynucleotide probes involved in the combination of pools of polynucleotide probe sets of said multiplicity of polynucleotide probe sets being such that each polynucleotide probe specifically hybridizes with one gene, and/or at least one of its variants when present, for determining the variation of expression at least 12 genes, and their variants when present, in order to diagnose the benign or malignant state of a breast tumor.

L'invention concerne l'utilisation d'une multiplicité d'ensembles de sondes polynucléotidiques, ladite multiplicité d'ensembles de sondes polynucléotidiques consistant en une combinaison de pools d'ensembles de sondes polynucléotidiques, chaque ensemble de sondes polynucléotidiques contenant au moins une sonde polynucléotidique choisie parmi une banque de séquences d'acides nucléiques, les sondes polynucléotidiques concernées dans la combinaison de pools d'ensembles de sondes polynucléotidiques de ladite multiplicité d'ensembles de sondes polynucléotidiques étant telles que chaque sonde polynucléotidique s'hybride spécifiquement avec un gène, et/ou au moins une de ses variantes le cas échéant, pour la détermination de la variation de l'expression d'au moins douze gènes, et leurs variantes lorsque présentes, pour diagnostiquer l'état bénin ou malin d'une tumeur du sein.

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