Molecular marker for muscle stem cells

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) A61K 48/00 (2006.01) C07K 14/82 (2006.01) C12N 5/06 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2285709

In accordance with the invention, Bcl-2 expression is a molecular marker for muscle stem cells. Thus, the invention provides method for identifying and isolating muscle stem cells. In addition, the invention provides methods for determining whether a test compound modulates muscle stem cell differentiation and/or proliferation. Finally, the invention provides methods for expressing an exogenous coding sequence in a muscle stem cell.

L'invention porte sur un procédé d'identification et d'isolement de cellules souches musculaires utilisant le marqueur moléculaire dit Bcl-2. L'invention porte également sur des procédés permettant de discerner si un composant de test module ou non la différenciation et/ou la prolifération de cellules souches musculaires. Elle porte en outre sur des méthodes d'expression d'une séquence de codage exogène dans une cellule souche musculaire.

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