Molybdenum transporter and gene thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 1/19 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2600565

It is intended to provide a novel molybdenum ion transporter MoTR1 gene which controls molybdenum transport for the first time in plants and can more efficiently promote environmental molybdenum uptake and molybdenum transport in vivo. By QTL analysis between Arabidopsis accessions Col-0 and Ler, it is found out that a QTL controlling the Mo concentration in leaves is located on the second chromosome. Using the gene analysis method, the region having a causative gene is limited to a region of 172 kb. In this region, there is a gene At2g25680 which has a domain common to a sulfate ion transporter but the function of which has never been clarified. Thus, two individual gene disruption lines having a foreign gene fraction (T-DNA) inserted into At2g25680 are obtained and Mo concentrations in leaves are measured, thereby identifying the MoTR1 gene.

L~invention concerne un nouveau gène MoTR1 transporteur d~ion molybdène qui contrôle le transport du molybdène pour la première fois chez les plantes et qui peut favoriser plus efficacement l~absorption et le transport de molybdène de l~environnement in vivo. A l~aide d~une analyse du locus quantitatif réalisée entre les accessions d~Arabidopsis Col-0 et Ler, il a été mis en évidence que le locus quantitatif contrôlant la concentration en Mo dans les feuilles est situé sur le second chromosome. A l~aide de la méthode d~analyse génétique, il a été déterminé que la région qui porte le gène responsable est limitée à une région de 172 kb. Dans cette région, un gène At2g25680 a un domaine commun avec celui d'un transporteur ion sulfate mais sa fonction n~a jamais été clairement établie. Ainsi, l~obtention de deux lignées de disruption génétique particulières possédant une fraction génétique étrangère (T-DNA) insérée au niveau de At2g25680 est réalisée et les concentrations en Mo dans les feuilles sont mesurées, permettant ainsi d~identifier le gène MoTR1.

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