Monitoring enzyme mixtures

Details Monitoring enzyme mixtures Monitoring enzyme mixtures

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12M 1/34 (2006.01)

Patent

CA 2641696

This invention provides a method for simultaneously detecting the presence of at least two enzymes in a sample, said method comprising the steps of; i) providing a first substrate for a first enzyme, said first substrate being labeled with a first fluorophore, ii) providing a second substrate for a second enzyme, said second substrate being labeled with a second fluorophore, iii) exposing the labeled substrates to the sample to allow the first and second enzymes present in the sample to interact with respective first and second fluorophore-labeled substrates to form respective first and second fluorophore- labeled substrate fragments; and, detecting the presence of said fluorophore-labeled substrate fragments.

L'invention concerne un procédé pour détecter simultanément la présence d'au moins deux enzymes dans un échantillon, le procédé consistant à : i) fournir un premier substrat destiné à une première enzyme et marqué avec un premier fluorophore; ii) fournir un second susbtrat destiné à une seconde enzyme et marqué avec un second fluorophore; iii) soumettre les substrats marqués à l'échantillon afin de permettre aux premières et aux secondes enzymes présentes dans l'échantillon d'interagir avec le premier et le second susbtrat marqués par fluorophore pour former des fragments respectifs des premier et second susbtrats marqués par fluorophore; et détecter la présence desdits fragments de susbtrat marqués par fluorophore.

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