Monitoring of cell adhesion and spreading

Details Monitoring of cell adhesion and spreading Monitoring of cell adhesion and spreading

C - Chemistry, Metallurgy

12

M

C12M 1/34 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2580548

The present invention includes devices and methods for dynamically monitoring cell adhesion and cell spreading. Cells are added to a microelectronic cell sensor array operably connected to an impedance analyzer. The device also includes a coating including biological molecule or organic compound capable of interacting with the cell. Cell adhesion and cell mobility is determined by detecting changes in impedance and comparing impedance or cell index values between samples.

La présente invention concerne des dispositifs et des méthodes de surveillance dynamique de l'adhésion cellulaire et de la diffusion cellulaire. Les cellules sont ajoutées dans un réseau de détection cellulaire microélectronique connecté à un analyseur d'impédance. Le dispositif comprend également un revêtement contenant une molécule biologique ou un composé organique pouvant interagir avec la cellule. L'adhésion cellulaire et la mobilité cellulaire sont déterminées par détection des changements d'impédance, et comparaison de l'impédance ou des valeurs d'indice cellulaire entre les échantillons.

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