Monitoring oligonucleotide binding processes using...

Details Monitoring oligonucleotide binding processes using... Monitoring oligonucleotide binding processes using...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2393575

Oligonucleotide building processes are monitored by means of an oligonucleotide probe which in one embodiment is labelled at one end with a chemiluminescent label and at the other end with a quencher molecule. The conformation of the oligonucleotide probe changes according to whether the probe hybridises with a substantially complementary nucleic acid sequence. In the non-hybridised state the chemiluminescent label is sufficiently close proximity to the quencher that the chemiluminescent emission is substantially attenuated, but in the hybridised state the separation is such that there is little or no attenuation. Particular probes and emitter/quencher pairs are disclosed.

La présente invention concerne la surveillance des processus de construction des oligonucléotides au moyen d'un oligonucléotide sonde qui, pour l'une des réalisations, est marqué en l'une de ses extrémités d'un marqueur chimio-luminescent, et à l'autre extrémité d'une molécule atténuatrice. La conformation de l'oligonucléotide sonde se modifie dans la mesure où la sonde s'hybride avec une séquence d'acide nucléique sensiblement complémentaire. A l'état non hybridé, le marqueur chimio-luminescent est suffisamment proche de l'atténuateur pour que l'émission chimio-luminescente s'atténue nettement, alors qu'à l'état hybridé, la séparation est telle qu'il n'y a pratiquement pas, voire absolument pas d'atténuation. L'invention concerne également des sondes particulières ainsi que des paires émetteur - atténuateur.

Affiliated with

Also associated with

Rating

Monitoring oligonucleotide binding processes using... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Monitoring oligonucleotide binding processes using..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Monitoring oligonucleotide binding processes using... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1452491