C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/7.4, 167/103
C07K 14/625 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1112639
Abstract of the Disclosure: The present invention relates to monocomponent insulin purified to such a degree that it shows only a single component when analyzed by amide gel electrophoresis using a gel containing 15% poly- acrylamide. It, furthermore, relates to a method of preparing such insulin from an insulin containing raw extract prepared from pancreas glands, consisting in the working up of the extract in such a manner that the insulin is maintained in dissolved state during the whole processing, without change of phase, until the final recovery of the insulin, the undesired substances being from the beginning of the process until the end removed from the different solvents used. A preferred method comprises removal of fat from the extract by cooling to a temperature below -25°C and separation of the crystallized fat, concentration of the extract and simultaneous removal of impurities present in the extract, both substances having a larger molecule than insulin and substances having a smaller molecule than insulin, by means of reverse osmosis plant for removal of colouring substances and salts, further purification by means of ion exchange and recovery of the insulin from the concentrated purified solution resulting from the ion exchange treatment by precipitation with metal ions under strong cooling. The monocomponent insulin may be used in insulin preparations of any kind for clinical use.
269730
Aguas Jose Adrian
Cebrian Gregorio Ramon
Jensen Willy Henry
Gowling Lafleur Henderson Llp
Laboratorios Leo S.a.
LandOfFree
Mono-insulin and method of preparing the same does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Mono-insulin and method of preparing the same, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Mono-insulin and method of preparing the same will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-322413