C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
167/37, 195/1.10
C12P 21/08 (2006.01) C07K 16/24 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1330768
ABSTRACT A novel monoclonal anti-human granulocyte colony stimulating factor (abbreviated as G-CSP) antibody, as well as a method of purifying human G-CSF by means of using said antibody and a method of assaying the same with said antibody. Said antibody was prepared by the following steps. A laboratory animal was immunized with human G-CSF derived from an oral cavity cancer serving as an antigen; spleen cells were extracted from the animal and fused with myeloma cells to prepare hybridomas; the hybridomas were treated to obtain clones that were capa- ble of producing a monoclonal anti-human G-CSF antibody in high concentrations; and the clones were cultivated to obtain a monoclonal anti-human G-CSF antibody from the supernatant of the culture.
524436
Imazeki Ikuo
Nomura Hitoshi
Chugai Seiyaku Kabushiki Kaisha
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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